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protein sample loading buffer (Epizyme Inc)

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Epizyme Inc protein sample loading buffer
Protein Sample Loading Buffer, supplied by Epizyme Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/loading+buffer/free+protein/pm42321161-53-13-19
Average 86 stars, based on 1 article reviews

protein sample loading buffer - by Bioz Stars, 2026-10

86/100 stars

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Article Title: MiR-1a-3p/Fcgr4-dependent osteoclast activation regulates pathological bone loss
Article Snippet: The supernatant protein samples were mixed with 5 × loading buffer (Epizyme, China; LT101), metal bath at 98 °C for 3 min and stored at -20 °C.

Article Title: Allicin-based biomimetic nanoparticles of the erythrocyte membrane for the delivery of lumefantrine to enhance its antimalarial effect
Article Snippet: The samples were mixed with 5× loading buffer (Epizyme Biotech, China) and heated at 100 °C for 10 min. Each sample was added to the gel wells after the aforementioned treatment.

Article Title: ZNF662 inhibits oncogenesis through NUPR1/p53 signaling pathway in epithelial ovarian cancer and is regulated by hsa-miR-429.
Article Snippet: A quarter volume of loading buffer (EpiZyme, Shanghai, China) was added, and samples were denatured by boiling at 100°C for 10 min.

Article Title: Chromatin and genomic instability in the cochlea contributing to age-related hearing loss: Insights from in vitro and in vivo models.
Article Snippet: Age-related hearing loss (ARHL) is one of the most common causes of hearing impairment in older adults.. However, the cellular and molecular mechanisms underlying ARHL remain unclear.. Chromatin and genomic instability, including DNA damage, heterochromatin loss, and telomere attrition, are hallmarks of aging.

Nucleic Acid Electrophoresis:

Article Title: A RIG-I targeting nanozyme induces PANoptosis for cancer immunotherapy.
Article Snippet: Protein extraction and western blotting (WB) Total protein was extracted with RIPA buffer (Thermo Fisher) supplemented with protease (Roche) and phosphatase inhibitors (Roche) on ice for 30 min, followed by centrifugation at 12,000 g at 4°C for 20 min. After sonication, the supernatant was quantified using the bicinchoninic acid (BCA) assay kit (Thermo Fisher Scientific). .. The protein solution was then boiled in loading buffer (Epizyme) for 10 min and the samples were run on a 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel (Epizyme). .. Following electrophoresis, the proteins were transferred onto 0.45 μm polyvinylidene fluoride (PVDF) membranes (Millipore).

SDS Page:

Article Title: A combinatorial therapeutic platform based on graphene-collagen cryogel and apoptotic vesicles for modulating hypertrophic scars.
Article Snippet: Cells were lysed in RIPA buffer (Epizyme, PC101) with protease inhibitors (Thermo ScientificTM, 78430; 4°C, 30min). .. After denaturation (100°C, 10min) with loading buffer (Epizyme, LT101), samples (20 μg/lane) were loaded onto the SDS-PAGE gel (FuturePAGE TM , ET15420LGel; 4-20% 15 wells) and transferred to PVDF membranes (AmershamTM, GE10600023) using a wet transfer system. .. Membranes were blocked with 5% non-fat milk (Epizyme, PS112), probed with primary antibodies (4°C, overnight) and HRP-conjugated secondary antibodies (RT, 1h), then detected with ECL (Epizyme, Omni-ECLTM SQ201).

Lysis:

Article Title: An orally bioavailable pan-αv/α5β1 integrin antagonist prevents aggressive prostate cancer progression via suppressing both oncogenic signals and CD47-mediated immune escape.
Article Snippet: In brief, cells or tumor tissue were lysed on ice with RIPA buffer (Solarbio, R0010, China) and centrifuged at 4 ̊C. .. After adding loading buffer (Epizyme, LT101, China), the lysis mixture was heated at 100 °C for 10 min. .. Equivalent amounts of protein were separated with 10-12% SDS-PAGE and transferred to PVDF membranes (Millipore, USA).

Membrane:

Article Title: Exploring the Therapeutic Effect of Polygonatum cyrtonema Polysaccharides in Reversing D-Galactose (D-Gal)-Mediated Cardiac Aging.
Article Snippet: After centrifugation (12,000× g, 10 min, 4 ◦C), the supernatant was transferred to a new centrifuge tube, and the protein concentrations were evaluated using a bicinchoninic acid (BCA) protein concentration assay kit (P0011, Beyotime, Shanghai, China). .. The proteins were added to 5× loading buffer (1LT101S, Epizyme, Shanghai, China) and denatured by heating at 100 ◦C for 10 min. A total of 30 μg of protein samples was separated on a 10% sodium dodecylsulfate polyacrylamide gel and transferred to polyvinylidene difluoride membrane (Millipore Corporation, Billerica, MA, USA). ..